h9c2 2 1 rat myoblasts Search Results


99
ATCC myoblast h9c2 2 1
Myoblast H9c2 2 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC embryonic rat
Embryonic Rat, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC rat heart ventricles
Rat Heart Ventricles, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
European Collection of Authenticated Cell Cultures h9c2 (2-1) rat db1x heart myoblast
H9c2 (2 1) Rat Db1x Heart Myoblast, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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95
ATCC 3t3-swiss albino
3t3 Swiss Albino, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC nih/3t3
Nih/3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caco-2  (ATCC)
99
ATCC caco-2
Caco 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l6  (ATCC)
96
ATCC l6
L6, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biowest SAS fetal bovine serum (fbs
Fetal Bovine Serum (Fbs, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Addgene inc rogfp2
The wound induced H 2 O 2 accumulation signal is accompanied by changes in cytosolic H 2 O 2 and redox levels. A . Monolayers of rat cardiomyocytes (H9c2) expressing <t>roGFP2-Orp1</t> grown in culture were wounded with a heated metal rod to induce injury and cytosolic H 2 O 2 levels were imaged as the ratio between 420/480 nm . Representative time-lapse images of whole plate changes in cytosolic H 2 O 2 levels in treated and untreated plates are shown alongside bar graphs of combined data from all plates used for the analysis at the 0- and 30-min time points (local and systemic; Background levels of cytosolic H 2 O 2 levels, measured at time 0, were subtracted from all other time points). B . Same as A, but for rat cardiomyocytes (H9c2) expressing roGFP2. Calibration of the roGFP2-Orp1 and roGFP2 signals in rat cardiomyocytes are shown in . All experiments were repeated at least 3 times with 10 plates per experiment. Data is presented in A and B as box plot graphs; X is mean ± S.E., N=30, **P < 0.01, ***P < 0.001, Student t-test. Scale bar, 1 cm.
Rogfp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h9c2+2+1+rat+myoblasts/pQE-60+roGFP2-His+(Plasmid+%2365046)/bio_rxiv__2023__12__19__572374-31-34-45
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90
JCRB Cell Bank gtl-16 cell line
The wound induced H 2 O 2 accumulation signal is accompanied by changes in cytosolic H 2 O 2 and redox levels. A . Monolayers of rat cardiomyocytes (H9c2) expressing <t>roGFP2-Orp1</t> grown in culture were wounded with a heated metal rod to induce injury and cytosolic H 2 O 2 levels were imaged as the ratio between 420/480 nm . Representative time-lapse images of whole plate changes in cytosolic H 2 O 2 levels in treated and untreated plates are shown alongside bar graphs of combined data from all plates used for the analysis at the 0- and 30-min time points (local and systemic; Background levels of cytosolic H 2 O 2 levels, measured at time 0, were subtracted from all other time points). B . Same as A, but for rat cardiomyocytes (H9c2) expressing roGFP2. Calibration of the roGFP2-Orp1 and roGFP2 signals in rat cardiomyocytes are shown in . All experiments were repeated at least 3 times with 10 plates per experiment. Data is presented in A and B as box plot graphs; X is mean ± S.E., N=30, **P < 0.01, ***P < 0.001, Student t-test. Scale bar, 1 cm.
Gtl 16 Cell Line, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Merck & Co dulbecco
The wound induced H 2 O 2 accumulation signal is accompanied by changes in cytosolic H 2 O 2 and redox levels. A . Monolayers of rat cardiomyocytes (H9c2) expressing <t>roGFP2-Orp1</t> grown in culture were wounded with a heated metal rod to induce injury and cytosolic H 2 O 2 levels were imaged as the ratio between 420/480 nm . Representative time-lapse images of whole plate changes in cytosolic H 2 O 2 levels in treated and untreated plates are shown alongside bar graphs of combined data from all plates used for the analysis at the 0- and 30-min time points (local and systemic; Background levels of cytosolic H 2 O 2 levels, measured at time 0, were subtracted from all other time points). B . Same as A, but for rat cardiomyocytes (H9c2) expressing roGFP2. Calibration of the roGFP2-Orp1 and roGFP2 signals in rat cardiomyocytes are shown in . All experiments were repeated at least 3 times with 10 plates per experiment. Data is presented in A and B as box plot graphs; X is mean ± S.E., N=30, **P < 0.01, ***P < 0.001, Student t-test. Scale bar, 1 cm.
Dulbecco, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The wound induced H 2 O 2 accumulation signal is accompanied by changes in cytosolic H 2 O 2 and redox levels. A . Monolayers of rat cardiomyocytes (H9c2) expressing roGFP2-Orp1 grown in culture were wounded with a heated metal rod to induce injury and cytosolic H 2 O 2 levels were imaged as the ratio between 420/480 nm . Representative time-lapse images of whole plate changes in cytosolic H 2 O 2 levels in treated and untreated plates are shown alongside bar graphs of combined data from all plates used for the analysis at the 0- and 30-min time points (local and systemic; Background levels of cytosolic H 2 O 2 levels, measured at time 0, were subtracted from all other time points). B . Same as A, but for rat cardiomyocytes (H9c2) expressing roGFP2. Calibration of the roGFP2-Orp1 and roGFP2 signals in rat cardiomyocytes are shown in . All experiments were repeated at least 3 times with 10 plates per experiment. Data is presented in A and B as box plot graphs; X is mean ± S.E., N=30, **P < 0.01, ***P < 0.001, Student t-test. Scale bar, 1 cm.

Journal: bioRxiv

Article Title: Propagation of a rapid cell-to-cell H 2 O 2 signal over long distances in a monolayer of cardiomyocyte cells

doi: 10.1101/2023.12.19.572374

Figure Lengend Snippet: The wound induced H 2 O 2 accumulation signal is accompanied by changes in cytosolic H 2 O 2 and redox levels. A . Monolayers of rat cardiomyocytes (H9c2) expressing roGFP2-Orp1 grown in culture were wounded with a heated metal rod to induce injury and cytosolic H 2 O 2 levels were imaged as the ratio between 420/480 nm . Representative time-lapse images of whole plate changes in cytosolic H 2 O 2 levels in treated and untreated plates are shown alongside bar graphs of combined data from all plates used for the analysis at the 0- and 30-min time points (local and systemic; Background levels of cytosolic H 2 O 2 levels, measured at time 0, were subtracted from all other time points). B . Same as A, but for rat cardiomyocytes (H9c2) expressing roGFP2. Calibration of the roGFP2-Orp1 and roGFP2 signals in rat cardiomyocytes are shown in . All experiments were repeated at least 3 times with 10 plates per experiment. Data is presented in A and B as box plot graphs; X is mean ± S.E., N=30, **P < 0.01, ***P < 0.001, Student t-test. Scale bar, 1 cm.

Article Snippet: H9c2(2-1) rat cardio myoblast (CRL-1446; ATCC) cells were plated at a density of 500,000/well for 6-well plates, and 25,000/well for 96-well plates and imaged using IVIS (Revvity) or Lionheart FX (BioTek), accordingly , . roGFP2 and roGFP2-Orp1 expressing cells were transfected with pMF1707 and pMOS016 (Addgene), accordingly, grown, and imaged as described previously , - .

Techniques: Expressing

Calibration measurements of rat cardiomyocytes (H9c2) expressing roGFP2-Orp1 ( A ) or roGFP2 ( B ) and treated with H 2 O 2 followed by DTT. After . Cells were imaged using an automated Lionheart FX (BioTek) fluorescence microscope. In support of .

Journal: bioRxiv

Article Title: Propagation of a rapid cell-to-cell H 2 O 2 signal over long distances in a monolayer of cardiomyocyte cells

doi: 10.1101/2023.12.19.572374

Figure Lengend Snippet: Calibration measurements of rat cardiomyocytes (H9c2) expressing roGFP2-Orp1 ( A ) or roGFP2 ( B ) and treated with H 2 O 2 followed by DTT. After . Cells were imaged using an automated Lionheart FX (BioTek) fluorescence microscope. In support of .

Article Snippet: H9c2(2-1) rat cardio myoblast (CRL-1446; ATCC) cells were plated at a density of 500,000/well for 6-well plates, and 25,000/well for 96-well plates and imaged using IVIS (Revvity) or Lionheart FX (BioTek), accordingly , . roGFP2 and roGFP2-Orp1 expressing cells were transfected with pMF1707 and pMOS016 (Addgene), accordingly, grown, and imaged as described previously , - .

Techniques: Expressing, Fluorescence, Microscopy